PROTECT is a platform technology that combines a precision blend of native microbiome-enhancing probiotics and prebiotics, working synergistically as a synbiotic to prevent infection and restore healthy microbiota. Probiotics consist of beneficial bacteria while prebiotics consist of nutrients that selectively enable growth of these beneficial bacteria. PROTECT is predicated on the concept of niche exclusion, allowing beneficial bacteria to outcompete the pathogens (such as Staphylococcus aureus and Pseudomonas aeruginosa (PA)) and preventing them from taking hold, spreading, or developing antibiotic resistance. The proposal is focused on systematically defining the appropriate bacteria and growth conditions for rational development of a synbiotic treatment to prevent PA infection in individuals with cystic fibrosis and other lung infections. Findings from this work could create a generalizable platform for producing protective agents against chronic and acute infections. The project intends to generate an integrated resource (the “Airway Systematic Microbial Atlas,” or ASMA). ASMA will store and present all data and analyses and link to the reagents developed (including a strain bank), providing the broader scientific and medical communities with unique data and material resources to extend analysis for novel applications.